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After solubilization with detergent, affinity purification and reconstitution in peptidiscs 32 , the protein was purified by size exclusion chromatography (Supplementary Fig

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Notably, gene-expression studies have associated GHK-Cu with the modulation of thousands of genes tied to tissue remodeling and the inflammatory response, which is why it is studied as a foundational signal peptide in repair models

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1 H-NMR (400 MHz, CDCl 3 ) ppm = 8.358.32 (m, AABB system, 2 H, Ar-H), 7.807.76 (m, 3 H, Ar-H), 7.707.65 (m, 2 H, Ar-H), 7.63 (dd, J = 0.6 Hz, J = 1.5 Hz, 1H, Ar-H), 7.527.46 (m, 2 H, Ar-H), 7.447.39 (m, 1H, Ar-H), 7.37 (dd, J = 1.5 Hz, J = 8.2 Hz, 1H, Ar-H), 3.50 (bs, 2 H, -CH 2 -), 3.23 (bs, 2 H, -CH 2 -), 1.7