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This work was supported by grants from the Hainan Province Clinical Medical Center (QWYH202175), the Scientific Research Project of Hainan Health Committee (21A200115), the Research and Cultivation Fund of Hainan Medical University (HYPY2020015), the Specific Research Fund of The Innovation Platform for Academicians of Hainan Province (YSPTZX202311), the Natural Science Foundation of Hainan Province (820RC771), and the Key R&D Projects of Hainan Province (ZDYF2022SHFZ074)

The morphological alterations exhibited in ferroptotic cells include obviously smaller mitochondria, increased membrane density of mitochondria, reduced inner mitochondrial membrane folds, and loss of structural integrity (Yagoda et al., 2007)

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Cell cytotoxicity assays A total of 1 10 5 cells/well in a 96-well plate were treated as indicated in figure legends or overexpressed with GSDME-NT