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If your vial accidentally froze (perhaps the refrigerator was set too cold or it was placed near the back wall of the fridge where temperatures can drop below freezing), do not use it

Standard laboratory handling practices typically include

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While it is not naturally produced, it is present in many foods including animal produce and seafood and can also be received through supplements

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Monitoring biofilm activity through tools like biofilm thickness sensors or regular measurement of BDOC (biodegradable dissolved organic carbon) can help operators ensure that the biofilm is functioning properly