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Some struggle in certain areas, while others have them more extensively

Biosci Biotechnol Biochem 80:540546

Hakola, L

Membranes were blocked with 5% skimmed milk in Tris-buffered saline containing 0.05% Tween 20 for 1 h at room temperature before overnight incubation at 4C with a 1:1,000 dilution of primary antibodies against total and phosphorylated AMPK and total and phosphorylated ACC (cat