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The analysis per mouse takes approximately 90 s and automatically calculated numerical results were analysed

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The 17-mer templates containing the adducts were paired with a 12-mer primer terminated with 2,3-dideoxyguanine (5-GGGGTGTGGTAG dd -3), and with dCTP as the incoming nucleotide

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Specifically, in male patients with GBA1 mutations, robust volcano-plot analysis revealed decreased levels of L-glutamic acid, L-alanine, choline, malonate, acetic acid, and pyruvic acid, coupled with upregulation of L-methionine and 3-hydroxybutyric acid compared with control subjects