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For the apo and P4S-bound states, conformational flexibility of TM1ainitially evidenced by discontinuous densitywas resolved through 3D variability analysis and focused 3D classification as described above, yielding four well-defined structures

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The same is observed if Dynabeads magnetic beads are exposed to DTT and EDTA

The amount of hydroquinone became too low to confer stabilizing activity and to interfere with our experiments 37

The Coenzyme A component requires pantothenic acid (Vitamin B5) for its formation