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Changes to the microbial phyla and genera observed in the 5A-1MQ-treated group switched from WD to LD could have been driven by additive, synergistic, or independent mechanisms relative to those induced by diet switch alone, which could not be determined with the current study design that lacked a group treated with 5A-1MQ and maintained on WD

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Researchers studying obesity, metabolic syndrome, or adipocyte biology often prefer NNMT inhibition over simple precursor supplementation because the effect is concentrated where it matters most

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