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Fluorescence microscopy was used to measure the longest DsRed-positive neurite (i.e., the axon) from YFP+ (ArfGAP1) or YFP- (empty vector) cortical neurons, as previously described [16]

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To dissect the upstream regulatory mechanism of miR-29b-3p under Gln deprivation conditions, we integrated transcriptome data (GSE148016) from both Gln+ and Gln- groups, and performed cross-analysis with the predicted upstream transcription factors of miR-29b-3p, ultimately identifying five candidate transcription factors

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