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This approach helps maintain treatment consistency and avoids missed doses

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For example, one donor may be more represented than the others in the microtissue, as we cannot control which donor cells attach best to form the microtissues in the OOC plates

J Ethnopharmacol 2025;120157

Huang C-J, et al

Fluorescent substrates (Dendra or sfGFP) containing the N-end rule degron and His14-SUMO tag were purified by Ni-NTA resin, followed by the SUMO tag cleavage, SUMO tag removal with Ni-NTA resin, and gel filtration, similarly to the purification of Ufd1 proteins