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Metabolomic studies have identified osmolytes and metabolites that play roles in maintaining cellular stability under these conditions

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The cell pellets were resuspended in lysis buffer (50 mM HEPES, pH = 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100 with protease inhibitors), incubated on nutator for 10 min at 4 C and centrifuged at 500 g for 5 min

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The NAPstar family allows the monitoring of NADP redox states across a 5000-fold range, spanning NADPH/NADP + ratios from approximately 0.001 to 5