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While this reaction initially required quite harsh acidic conditions (pH 3), it was subsequently found that this was primarily to denature early protein substrates and hence to expose the reactive tryptophan residue

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10.1128/IAI.00641-12 51

When the expression of Nrf2/ARE is at zero circadian time point, expression of glutamate-cysteine ligase modifier (GCLM), NAD(P)H dehydrogenase [quinone] 1 (NQO1), and heme oxygenase 1 (HMOX1) proteins are weakened, resulting in severe oxidative injury (Tonelli et al., 2018

doi:10.1016/j.parkreldis.2024.107015

It is also taken by mouth to prevent cancer, including breast cancer, cervical cancer, colorectal cancer, and lung cancer