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Subsequently, added 60 L of 50% acetonitrile, vortexed with shaking at 1700 rpm for 10 minutes at 8 C, centrifuged at 15,000 g for 20 minutes at 4 C, and aspirated the supernatant and stored at 80 C until analysis

doi: 10.1038/s41590-022-01219-w 176 JadhavRRImSJHuBHashimotoMLiPLinJXet al

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Add bacteriostatic water slowly down the side of the vial and swirl gently until dissolved

In Japan, treating multiple sclerosis patients with daily 60,000g tablets of methyl B12, for six months, proved to be useful and safe