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Western blot assay K7M2 cells were incubated with the PBS, Ru(II)-OH, NP2, or NP4 (10 M Ru) for 12 h at 37 C, and the supernatant was subsequently co-incubated with RAW264.7 cells

The elabela-APJ axis could mitigate neuronal ferroptosis following ischemic stroke, and the ELA-32 peptide may be a potential therapeutic candidate for ischemic stroke and cardiovascular diseases

Blots were blocked with 5% milk in PBS-T (0.01% Tween-20 in phosphate buffered saline)

Medications: Some medications, such as hormone replacement therapy, chemotherapy and certain antibiotics, can increase sensitivity to sunlight and cause pigmentation as a side effect

Therefore, de novo synthesis of GSH is essential for the adaptive response to oxidative stress

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