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There are some proteins, he asserts, that are better expressed and more soluble when they carry natural polyhistidine tagging compared with the artificial hexahis tag. Additionally, the energy requirements for an expressing organism like Escherichia coli to add six histidine residues during translation are higher than for those naturally occurring sequences

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Stato Clinico: RCT umano | Osservazionale | Animale | In vitro Studiato principalmente in contesti di ricerca preclinica ed esplorativa umana sulla modulazione dellormone della crescita

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For each sample, 128 free induction decays (FID) were collected using 32k data points within 10 ppm, and the total data collection time was 11 min

Nature 523 , 617620 (2015)