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Vitamin B12 is one of eight B vitamins

NNMT (nicotinamide N-methyltransferase) is a cytosolic enzyme that catalyzes a specific methylation reaction: it transfers a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing two products 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH)

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ImageJ software version 1.52a (Wayne Rasband, National Institutes of Health, United States) was used to quantify the mesangial area and analyze Masson intensity