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Plasmid transfection and luciferase reporter assay The luciferase-based reporter plasmids pNFKB-Luc and pTA-Luc (Takara Bio, San Jose, USA) were applied with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturers instructions

Because its endogenously produced rather than purely synthetic, researchers frequently compare it with lab-synthesized copper-peptide analogs to assess how closely manufactured versions replicate the naturally occurring molecules structure and behavior

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The simplified 2D microenvironmentdevoid of a 3D matrix, vasculature, and key factors such as CPfurther leads to dysregulated copper transporter expression and non-physiological toxicity responses [252]

In a mechanism-based manner, TGF- linked to the membrane of PCa stimulates EGFR on osteoblasts through cell-to-cell adhesion during bone metastasis (Fig