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Studies have shown that mitochondrial dysfunction often leads to excessive ROS production, resulting in genomic instability, cancer progression, and metastasis

[DOI] [PMC free article] [PubMed] [Google Scholar] 151.Bodke H., Jogdand S

Cells 9 , 2418 (2020)

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62 , e202219009 (2023)

To assess whether both sites are required for Nedd4-2 membrane binding, we replaced these residues and M122 (control) with leucine by site-directed mutagenesis and examined the structural integrity of the mutants by Differential scanning fluorimetry (DSF) and circular dichroism (CD) spectroscopy