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For instance, the selection of the ITS1 versus ITS2 region for amplicon sequencing can lead to differential amplification efficiencies and taxonomic biases, with ITS1 primers often favoring Ascomycota and ITS2 better capturing Basidiomycota

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In pancreatic cancer cells, oncogenic K-Ras maintains the nucleotide pool via the MAPK-dependent signaling pathway, leading to MYC upregulation, and the use of MEK inhibitors reduces the incorporation of glutamine-derived nitrogen into purine nucleotides 38