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Tissue processing Following the 3 weeks of cuprizone treatment, mice were euthanised using pentobarbitone sodium (160 mg/kg, Delvet), and transcardially perfused with 0.9% saline, followed by 4% paraformaldehyde (Sigma-Aldrich) in 0.1 M phosphate buffered solution pH 7.4

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Doctors often use Phentermine before resorting to surgical intervention, due to its proven safety and efficiency

Based on the outcome of the BLOOM (46) and BLOSSOM trials (47), in 2012 the FDA approved lorcaserin as an addition to a reduced-calorie diet and exercise for eligible patients (48)

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