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Aging Cell, 20(1), e13292

The WT H1.4 coding sequence was amplified using primers 5- GGTTCTGGTGGTGGTTCGATGTCCGAGACTGCGCCT and 5- GGTACCGTCGACTGCAGCTACTTTTTCTTGGCTGCC and cloned into EcoRI-linearized C1L by Gibson assembly

Besides control groups, hyperthyroidism was triggered in the rats by 100 g/kg of l-thyroxin and further treated with quercetin (QT) and nano-quercetin (10 mg/kg body weight)

In brief, lipidomics for each sample were performed by the Vanquish UPLC systems coupled to a Q Exactive HF (QE-HF) mass spectrometer equipped with HESI (Thermo Fisher Scientific, Waltham, MA)

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