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Several factors can contribute to the progression of cancer, such as exposure to the risk factors, abnormal expression and degradation of oncoproteins and tumor suppressor proteins, abnormal DNA damage repair, and changes in metabolic enzyme activity [3,4,5]

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The column was then washed with buffer A (300 L, 2,000 g , 2 min), and 10 mM aqueous NH 4 HCO 3 containing 5% CH 3 CN (300 L, 2000 g , 2 min), before peptides were eluted from the column as 15 fractions using 300 L buffer containing an increasing concentration of CH 3 CN in 10 mM NH 4 HCO 3 (%CH 3 CN = 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5, 30, 35, 40, 45, 50, 75) (2000 g , 2 min)

doi: 10.1111/1462-2920.13589

Quantification of Iba1 + cells was performed using ImageJ software, with results expressed as fluorescence intensity per mm 2 or mean fluorescence intensity (MFI)

Replace bacteriostatic water every 28 days