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available at www.novocraft.com), ELAND (Illumina a guide sequence within a nucleic acid-targeting guide RNA a guide sequence may direct sequence-specific binding of a nucleic acid -targeting complex to a target nucleic acid sequence the components of a nucleic acid-targeting CRISPR system sufficient to form a nucleic acid-targeting complex, including the guide sequence to be tested, may be provided to a host cell having the corresponding target nucleic acid sequence, such as by transfection with vectors encoding the components of the nucleic acid-targeting complex, followed by an assessment of preferential targeting (e.g., cleavage) within the target nucleic acid sequence, such as by Surveyor assay as described herein