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R.Amini-KhoeiH.et al (2024)

Th17/Treg cells imbalance and their related cytokines (IL-17, IL-10 and TGF-) in children with autism spectrum disorder

Western blotting Cells were lysed in modified RIPA buffer (50 mM TrisHCl (pH 7.5), 150 mM NaCl, 10 mM -glycerophosphate, 1% NP-40, 0.25% sodium deoxycholate, 10 mM sodium pyrophosphate, 30 mM sodium fluoride, 1 mM EDTA, 1 mM vanadate, 20 mg/ml aprotinin, 20 mg/ml leupeptin, and 1mM phenylmethylsulfonyl fluoride)

Levels of the ROS on the LR-RBCs As the RBCs expose to the ROS, their quality decrease

It was noted that the surface modification by several compounds helps to give better repulsion with the blood components, allow more drug loading capacity, and enable more circulation time [23,24]

Zhang et al., 2024