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Following the run, slides were put in a neutralization buffer (0.4 M Tris, pH 7.5) 3 times for 5 min

Modeling mechanical activation of macrophages during pulmonary fibrogenesis for targeted anti-fibrosis therapy

When adopting nanocarriers, developers must assess carrier-driven immunogenicity, stability, and scalable manufacturing approaches (Zhang et al., 2024

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The addition of butyric acid led to a decrease in FOSL2 m6A methylation level and mRNA expression through inhibition of METTL3 expression and was accompanied by a decrease in NLRP3 and inflammatory factors IL-6 and TNF- expression, whereas FOSL2, as an AP1 family member, participates in the immune response and promotes the expression of inflammatory factors (229)

For FISH assay, 18S rRNA probe was used as positive control