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These metabolic alterations are not only critical for defining different T cell subsets, but also for sustaining their activity in inflammatory environments

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The vesicle-associated radioactivity was determined by liquid scintillation counter

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Silver staining Following SDS-PAGE, gels were silver stained by immersing them in 50% methanol and 10% acetic acid for 30 min, then in 5% methanol for 15 min, and finally washing three times with ultrapure water (5 min each)

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