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The appropriate buffer was then used to dissolve SAM from stocks to 500 M

Since thioredoxin is readily oxidized, it has to be reduced using thioredoxin reductase before it is used for experiments

This is why some people say, Boiled water tastes better, while others say, Boiled water tastes flat or worse. Taste depends on your water chemistry, your plumbing, and whether boiling concentrates minerals