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The absorbance was measured at 517 nm against a blank through a microplate ELISA reader (Bio Tek, United States)

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145 Under oxidative stress, Cys34 thiol shifts to a sulfenic acid (HSA-SOH), which can be irreversibly oxidized to the sulfinic (HSA-SO2H) and sulfonic (HSA-SO3H) acids or, through the reactions with glutathione, can be returned to the HSA-SH reduced form