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qPCR (20 L) was carried out by using 2 Ultra SYBR Mixture (CWbio, China) in 12 8 well plates in a CFX Connect Real-Time System (Bio-Rad), containing 10 L of SYBR Mixture, 2 L cDNA, 1.6 L of qPCR primers (4 M), and 7.4 L nuclease-free water

Taken together, our findings strongly suggest exposure to PDH and thus, the pyruvate-depletion induced dispersion response, to require LdhA and MifR

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[19] [21] The duration of therapy depends on the indication and patient response

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