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After two freeze-thaw cycles, 20 L of 5% 5-sulfosalicylic acid (SSA) was added, followed by centrifugation at 8,000g for 10 min

It also helps to understand why the three arms were combined in the first place

Most of the mammalian GMB is formed by the Bacteroides genus of Gram-negative, obligate anaerobes within the Bacteroidota phylum (formerly known as Bacteroidetes )

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