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The single cells and the cell spheroids used in the experiments are first prepared by culturing cells in conventional monolayer and 3D spheroid formats as shown in Figure 1A

Nature 491 , 384392

These mechanisms include interaction with the electron transport chain, through a pathway of oxidative phosphorylation, or blocking a nutrient uptake

TIM3 Mediates T Cell Exhaustion during Mycobacterium tuberculosis Infection

Microscopically, the group treated with boswellic acid indicated an intact cellular framework along with no apparent signs of inflammation, showing the potent anti-inflammatory activity of boswellic acid