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After taking an aliquot for protein estimation, worms were resuspended in 100 l of H 2 O and 230 l of Eluent A (95% acetonitrile, 0.1 mM ammonium acetate and 0.01% NH 4 OH) was added together with 1 l of 1 M chloropropamide as internal standard

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Appropriate reconstitution procedures contain gently swirling or rolling the vial to dissolve the peptide powder with out vigorous shaking to keep away from degradation