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The precleared lysates were incubated with antibodies against SNARE proteins (Syn4, SNAP-23, VAMP2, and VAMP8) for 2 h at 4C for binding, and then the antibodyantigen complexes were precipitated by incubation with a protein G slurry overnight with constant shaking

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If a patient has persistent mild to moderate hemolytic anemia, despite receiving appropriate therapy, you should consider the possibility of inborn errors of metabolism

doi: 10.1016/j.cegh.2024.101663 24 JarczakD.KlugeS.NierhausA

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