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Several groups have applied single-nucleus transcriptomics (snRNA-seq) to ventral tegmental area (VTA) neurons and glial cells across three stagespre-addiction, addiction, and post-addictionyielding deeper insights into nicotine-induced changes (Scott et al., 2024), proteomics ( 5 Conclusion Nicotine addiction arises from the diversity and dynamic regulation of nAChR subtypes, which shape the balance between rewards and aversion in mesolimbic circuits

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Mechanistic view of hnRNPA2 low-complexity domain structure, interactions, and phase separation altered by mutation and arginine methylation

However, biological effects can extend far beyond the detection window

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