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Proteins were extracted from 5 to 50 ml of exponentially growing suspension culture (OD 600 =0.81.2) by centrifugation and grinding with glass beads (5 min, IKAVibrax-VXR, Janke und Kunkel) at 4 C in 150300 l Native lysis buffer (50 mM Na-HEPES (pH 7.5)

Research suggests DSIP may promote slow-wave (deep) sleep, which supports recovery, hormone regulation, and cognitive function

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It is one of the most important antioxidants in the body

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They typically contain reactive functional groups, such as amines, thiols, or carboxyls, which allow selective linkage between different molecules for targeted drug delivery, fluorescent labeling, or functional modifications