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for 6 min, following which the reaction was terminated by the addition of a mixture of 10% trichloroacetic acid and 4% methanol, vortexing for 5 s, and centrifuging at 14,000 rpm for 2 min to precipitate the protein

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If your compounded semaglutide arrives as a liquid at a specific concentration (for example, 2.5mg/mL in a 2mL vial), skip the reconstitution step entirely and proceed directly to dose calculation based on the labeled concentration