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Why the Glp pathway, and presumably MGO production, is upregulated in the presence of GO remains to be deciphered

Cells or organoids were solubilized in pull-down buffer (50 mM Tris pH 7.3, 150 mM NaCl, 1% Triton X-100, 0.1 mM CaCl 2 plus protease/phosphatase inhibitors as above) and centrifuged at 14,000 rpm for 10 min at 4C

Litwack, G., B

2023 Feb 3;24(3):2959

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This target-centric research approach has provided valuable insights into the precise disruption of bacterial cell functions by antibiotics (Sodhi et al., 2023), an excessive reliance on this singular perspective also imposes certain limitations