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New controversies have also emerged, such as the question of whether microglia are active or reactive players in neurodegenerative disease conditions, or whether they may be victims themselves [185]

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However, ubiquitin C-terminal 7-amido-4-methylcoumarin (Ub-AMC) and its analogs, due to their high fluorescence sensitivity and hydrolysis efficiency, are often used to quantitatively determine the activities of DUBs without considering their substrate specificities (8,9)

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J Biosci Bioeng 123(5):540546 Overman RC, Debreczeni JE, Truman CM, McAlister MS, Attwood TK (2014) Completing the structural family portrait of the human EphB tyrosine kinase domains