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Trizol RNA isolation reagent (Gibco BRL, Grand Island, USA) was used to extract sperm RNA following manufacturers instructions

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If you are applying it topically, do a patch test to avoid any adverse side effects on your skin

Saudi J Biol Sci (2020) 27(10):25636

In certain embodiments these compounds contain a methyltrisulfide that can be reacted with appropriate thiols to form disulfides, at the same time introducing a functional group such as a hydrazide or other functional group that is useful for conjugating calicheamicin to an anti-ALPPL2 antibody