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The thermal cycling was run using the BioRad CFX96 Real Time Detection System programmed as follows: one cycle of heating at 95C for 3 min, 36 cycles of denaturation at 93C for 30 s, annealing at 62C for 45 s, and elongation at 72C for 40 s

[DOI] [PMC free article] [PubMed] [Google Scholar] 725.Shackleton M, Quintana E, Fearon ER, Morrison SJ