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In the next step, glass slides were incubated with goat anti-rabbit IgG (H+L) HRP secondary polyclonal antibody (1:1000, Yeason) at 37C for 1 h and then with DAB for approximately 5 min

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It's a more upstream, foundational approach, influencing the very 'engine' of the cell

NAD+ enhancement has been shown to reduce neuroinflammation and improve cognition in models of neurodegeneration [3]