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The advantage of TAP-VK1 was that the reaction mixture of thiol-labeling could be directly used for functional analyses without the isolation and purification of the labeled substrate, because the reaction in sodium phosphate buffer (pH 7.0) did not require any reagents other than 2a , and excess 2a did not disturb the fluorescence observation and did not show cytotoxicity, unlike other compact fluorescent labeling reagents

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V., Archontaki, H

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All other chemicals used in our experiment were of analytical grade