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Secondly, treatment of BMECs with MGO leads to a decrease in phosphorylated protein kinase B (Akt) to non-phosphorylated Akt, part of the phosphoinositide 3-kinases/Akt (PI3K/Akt) pathway [64, 74]

To assess enzyme activity, the amount of H 2 O 2 consumed over a specific time was quantified alongside a standard curve

10.1038/s41418-019-0488-1 14 DasI.TuominenR.HelledayT.HanssonJ.Warpman BerglundU.Egyhazi BrageS

Energy Expenditure and Thermogenesis GLP3s glucagon receptor activation specifically contributes to increased energy expenditure through multiple pathways[6]: Enhanced hepatic fatty acid oxidation and lipolysis Increased thermogenic activity in brown adipose tissue Upregulation of uncoupling protein expression Potential FGF21-mediated metabolic effects This thermogenic component distinguishes the blend from pure GLP-1 or amylin-based approaches, addressing both energy intake (via satiety) and energy expenditure simultaneously

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