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By binding to proteins, such as transferrin, Fe 3+ can be transported in a redox-inactive state

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To prevent non-specific antibody adsorption, the cells were blocked in PBS containing 10% FBS at room temperature for 1 h before being incubated overnight at 4 C with the appropriate primary antibodies Following 3 washes with PBS, the cells were incubated at room temperature for 1 h with secondary antibodies, followed by 3 additional washes with PBS