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Step 2: The transsulfuration pathway Next, methionine enters the transsulfuration pathway to produce cysteine , the rate-limiting amino acid for glutathione

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Erastin inhibits system Xc-, decreasing GSH uptake and GPX4 activity, ultimately triggering ferroptosis by lowering cell antioxidant defenses and increasing lipid ROS

NF-B activation mediated by ER stress is dependent on Ca 2+ efflux and subsequent production of reactive oxygen species (ROS) ( The NF-B and ER stress in atherosclerosis Three stages of atherosclerosis progression Atherosclerosis is a common chronic inflammatory disease characterized by the accumulation of fibrin and lipids in subendothelial space, being a leading cause of cardiovascular diseases, including heart failure, stroke, and claudication (53, 54)

Nevertheless, although it is generally accepted that reactive metabolite formation plays an important role in AQ-induced toxicity (Srivastava et al., 2010