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Animal experiments All animal procedures were performed in accordance with the ethical standards approved by the Animal Care and Ethics Committee of the Second Affiliated Hospital, Zhejiang University School of Medicine

This was further substantiated by the observation that inhibiting heightened GJ function with a GJ inhibitor in mice effectively diminished APAP toxicity, which correlated with reduced levels of oxidative stress in hepatocytes

Additionally, we harvested and immortalized the stromal vascular fraction (SVF) from the inguinal WAT of Slc25a45 flox/flox , from which we established SLC25A45 KO cells and control cells by infecting immortalized SVFs with a retroviral Cre (pMSCV-Cre) or empty vector as control ( Fig

2, 5, 6), the calculated binding free energy again indicates the rapid oxidation of C3G against EC and CH compounds

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Study on the interaction between active components from traditional Chinese medicine and plasma proteins