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Pulldown assays were performed using lysates derived from either RAW264.7 macrophages, where Rab12 is phosphorylated by LRRK2 under basal conditions, in the absence and presence of GSK2578215A, to inhibit phosphorylation by LRRK2, or from untreated RBL cells, in which Rab12 is non-phosphorylated, or Ion/TPA-activated RBL cells, in which Rab12 is robustly phosphorylated by LRRK1

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The mean values for troponin were 5.5 0.51, 28 1.7, 14 1.2, and 7.6 0.96

Darwin, K