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2.4 Blood metabolism parameters and blood count Blood samples were collected from calves by jugular puncture on the first day, 40 and 60 days of the experiment, two hours after offering the milk replacer, for analysis of selenium, iron, blood count, ferritin, and the following biochemical parameters: -hydroxybutyrate, glucose, total protein, urea, creatinine, creatinine kinase, lactate dehydrogenase (LDH), lactate, albumin, and IgG