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The forward and reverse oligonucleotides of this fragment were, respectively, 5- GATCT ATGCTGTTTAATCTGAGGATCCTGTTAAACAATGCAGCTTTTAGAAATGGTCACA ACTTCATGGTTCGAAATTTTCGGTGTGGACAACCACTACAAG-3 and 5- AATTC TTGTAGTGGTTGTCCACACCGAAAATTTCGAACCATGAAGTTGTGACCATTTCTA AAAGCTGCATTGTTTAACAGGATCCTCAGATTAAACAGCATA-3 ( Bgl II and Eco RI sites underlined