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The axiom - which extends to blood levels - cannot be overstated, while appreciating the difficulty in estimating that load (21, 111)

The cells were plated in complete DMEM medium at a density of 1 10 4 cells per well in 96-well black microplates with clear bottom and after 24 h incubated with 40 nM DiOC6 in PBS for 20 min at 37 C and 5% CO 2 in the dark

J Neurosci 19:562569 Valdovinos-Flores C, Gonsebatt ME (2012) The role of amino acid transporters in GSH synthesis in the blood-brain barrier and central nervous system

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Interaction with chelators avoid concurrent use of EDTA or other strong chelators, which may strip copper from the peptide complex and abolish activity