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To calculate the specific activity of GPx (in micromoles of GSH per minute per 1 mg of protein), the decrease in GSH concentration in enzymatic oxidation over 1 min (mol/min/ml) was divided by the protein concentration in the reaction mixture (mg/ml)

also observed with IV administration in humans as shown by an increase in inflammatory markers

May 26 2021;22(11)doi:10.3390/ijms22115644

However, those with AMC were older (69.4 4.3 vs 63.3 7.5 yrs , p = 1.35 (75% vs 52%, p =0.046)

Photobiomodulation in dental implant stability and post-surgical healing and inflammation

As above, the microneedling rationale rests on studies of other compounds (PMID 23960389, PMID 40225275), not on AHK-Cu data